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ED4SS

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

99.0-102.0% (titration)

Sinónimos:

EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate

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Fórmula lineal:
(NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O
Número CAS:
10378-23-1
Peso molecular:
416.20
NACRES:
NA.77
PubChem Substance ID:
24894711
EC Number:
200-573-9
Beilstein/REAXYS Number:
3861753
MDL number:
MFCD00150026
UNSPSC Code:
12352116
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Quality Level

300

assay

99.0-102.0% (titration)

form

crystalline

reaction suitability

reagent type: chelator

solubility

H2O: soluble, clear, colorless

SMILES string

O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4

InChI key

KSYNLCYTMRMCGG-UHFFFAOYSA-J

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid and a metal chelating agent. It comprises four carboxylic acid and two amine groups with lone-pair electrons that can chelate calcium and several other metal ions. 

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:
  • in the decalcification of femurs for the histoprocessing and staining of osteochondral tissue
  • as a component of NH4Cl lysis buffer for erythrocyte lysis and bulk characterization
  • for the isolation of crypts from the connective tissues of mice

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.
Ethylenediaminetetraacetic acid (EDTA) is used to treat patients poisoned with heavy metal ions. It functions as a chelator of the zinc ion in the active site of metalloproteases. EDTA inhibits other metal ion-dependent proteases such as calcium-dependent cysteine proteases. It might interfere with biological events which are metal ion-dependent. EDTA inhibits platelet aggregation and is the most preferred anticoagulant for platelet counts. It associates with other active agents to modulate microorganisms and biofilms, like citric acid, alcohol, antibiotics, polyhexamethylene biguanide (PHMB) quaternary ammonium compounds, and other antiseptics. EDTA is used as an anticoagulant for hematological testing, as it preserves the cellular components and morphology of blood cells. 

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Este artículo
E65110369903699
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate 99.0-102.0% (titration)

Sigma-Aldrich

ED4SS

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate BioReagent, suitable for cell culture, 98.5-102.0%

Sigma-Aldrich

E6511

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

Ethylenediaminetetraacetic acid tetrasodium salt hydrate BioUltra, ≥99.0% (KT)

Sigma-Aldrich

03699

Ethylenediaminetetraacetic acid tetrasodium salt hydrate

Ethylenediaminetetraacetic acid tetrasodium salt hydrate BioUltra, ≥99.0% (KT)

Sigma-Aldrich

03699

Ethylenediaminetetraacetic acid tetrasodium salt hydrate

assay

99.0-102.0% (titration)

assay

98.5-102.0%

assay

≥99.0% (KT)

assay

≥99.0% (KT)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

solubility

H2O: soluble, clear, colorless

solubility

H2O: 160 mg/mL

solubility

H2O: 0.1 M at 20 °C, clear, colorless

solubility

H2O: 0.1 M at 20 °C, clear, colorless

form

crystalline

form

powder

form

powder

form

powder

reaction suitability

reagent type: chelator

reaction suitability

-

reaction suitability

reagent type: chelator

reaction suitability

reagent type: chelator

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Respiratory Tract

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000559417
0000543936
0000534652
0000534653
0000525166

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Número de artículo de comercio global

SKUGTIN
ED4SS-100G04061833609361
ED4SS-2.5KG04061833609385
ED4SS-500G04061835544837
ED4SS-1KG04061833609378

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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