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E6779

Millipore

EZview™ Red Anti-HA Affinity Gel

Sinónimos:

Anti-HA

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

shelf life

1 yr at 2‑8 °C

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

crosslinked agarose

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

storage temp.

2-8°C

General description

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Specificity

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Application

EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Other Notes

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Related product

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Novel Mitochondrial Substrates of Omi Indicate a New Regulatory Role in Neurodegenerative Disorders
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Gao S, et al.
The Journal of biological chemistry, 292(9), 3683-3691 (2017)
The nonreceptor tyrosine kinase c-Abl phosphorylates Runx1 and regulates Runx1-mediated megakaryocyte maturation
Liu H, et al.
Biochimica et biophysica acta. General subjects, 1865(8), 1060-1072 (2018)
The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis.
Chen YL, Capeyrou R, Humbert O, et al.
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Hadel Alsaran et al.
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