DPBS and other basal salt solutions are multi-component formulations. In total, this product requires 9.6 g to prepare 1L of a 1X solution. A molar concentration will not apply to the entire final product, only to the concentration of the individual components. See the link below for a formulation table for the DPBS products. The concentration of each component in these formulations are listed:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/354/091/d5773for.pdf
D5773
Disolución salina tamponada con fosfatos de Dulbecco
Without calcium chloride, powder, suitable for cell culture
Sinónimos:
DPBS
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Use this formulation of DPBS when tissue or cell dissociation or release is the objective. Also, suitable for studies that involve calcium metabolism.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
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| description Without calcium chloride, powder, suitable for cell culture | description liquid, sterile-filtered, with calcium, with magnesium, suitable for cell culture | description With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | description Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, 0.1 μm filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 200 |
| form powder | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C |
DPBS 1L for research | 4′,4′′-Methylenebis(2-chloroacetanilide) ACS grade, 4′,4′′-Methylenebis(2-chloroacetanilide) application in organic synthesis, 4′,4′′-Methylenebis(2-chloroacetanilide) chemical, 4′,4′′-Methylenebis(2-chloroacetanilide) for synthesis, 4′,4′′-Methylenebis(2-chloroacetanilide) procurement, 4′,4′′-Methylenebis(2-chloroacetanilide) reagent for titration, 4′,4′′-Methylenebis(2-chloroacetanilide) safety data sheet (SDS), 4′,4′′-Methylenebis(2-chloroacetanilide) technical data sheet, DPBS, Isotonic saline, cell washing solution, cell suspension solution, Sigma-Aldrich 4′,4′′-Methylenebis(2-chloroacetanilide) for research, high purity 4′,4′′-Methylenebis(2-chloroacetanilide), laboratory-grade 4′,4′′-Methylenebis(2-chloroacetanilide), ordering 4′,4′′-Methylenebis(2-chloroacetanilide) in | DPBS, Isotonic saline, cell washing solution, cell suspension solution | 500mL DPBS with inorganic ions, DPBS solution for mammalian cell research, DPBS suitable for cell culture applications, DPBS with antibiotics for mammalian cell culture, DPBS, Isotonic saline, cell washing solution, cell suspension solution, Dulbecco phosphate buffered saline 500mL, Dulbecco phosphate buffered saline for microtubule detection, Dulbecco phosphate buffered saline with glucose 500mL, Dulbecco′s phosphate buffered saline for cell recovery, Dulbecco′s phosphate buffered saline for ultrasound drug delivery studies, Sigma DPBS for laboratory use, Sigma-Aldrich DPBS cell culture solution, isotonic Dulbecco′s phosphate buffered saline for primary cells, lab-grade DPBS for contamination-sensitive applications |
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13 - Non Combustible Solids
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Hello, it is my first time buying DPBS powder. May I know how much should I weigh to prepare 1M of solution? My product is D5773-10L. Thank you.
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