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D1538

Sigma-Aldrich

Piperazine diacrylamide

For acrylamide gel electrophoresis

Sinónimos:

1,4-Bis(acryloyl)piperazine, 1,4-Di(acryloyl)piperazine, N,N′-Bis(acryloyl)piperazine, BAP, PDA, Piperazine diacrylamide

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About This Item

Fórmula empírica (notación de Hill):
C10H14N2O2
Número de CAS:
6342-17-2
Peso molecular:
194.23
Beilstein/REAXYS Number:
745431
MDL number:
MFCD00077661
UNSPSC Code:
12352200
PubChem Substance ID:
24893477
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

mp

91.5-93.5 °C (lit.)

storage temp.

room temp

SMILES string

C=CC(=O)N1CCN(CC1)C(=O)C=C

InChI

1S/C10H14N2O2/c1-3-9(13)11-5-7-12(8-6-11)10(14)4-2/h3-4H,1-2,5-8H2

InChI key

YERHJBPPDGHCRJ-UHFFFAOYSA-N

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General description

1,4-Bis(acryloyl)piperazine is a reagent that can be substituted for bis-acrylamide in the preparation of polyacrylamide gels.

Application

Suitable for preparation of gels for PAGE, IEF, 2D electrophoresis and protein sequencing.

Features and Benefits

  • increases the gel strength and resolution
  • reduces background for silver background
  • can be substituted for bisacrylamide without changing polymerization protocol

related product

Referencia del producto
Descripción
Precios

P7170

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

P7059

Disolución salina tamponada con fosfato, 10× PBS for Western blots and IP

B5655

SIGMAFAST™ BCIP®/NBT, tablet

B6404

BCIP®/NBT solution, premixed

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

W0138

Western Blocker™ Solution, for HRP detection systems

SDS7B2

Prestained Molecular Weight Marker, mol wt 26,600-180,000 Da

C1992

Marcador de electroforesis ColorBurst™, mol wt 8,000-220,000 Da

GERPN2209

ECL™ Western Blotting Detection Reagents, Cytiva RPN2209

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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G Artoni et al.
Analytical biochemistry, 137(2), 420-428 (1984-03-01)
The properties of gels prepared either from acryloyl-morpholine (ACM) or from its mixtures with acrylamide and crosslinked either with bisacrylylpiperazine or with methylenebisacrylamide have been described. ACM-containing gels are compatible with organic solvents. If polymerized in water and dried, they
Su-Hyung Hong et al.
Biomarker insights, 1, 175-183 (2008-09-05)
Pancreatic cancer has a poor prognosis, in part due to lack of early detection. The identification of circulating tumor antigens or their related autoantibodies provides a means for early cancer diagnosis. We have used a proteomic approach to identify proteins
Ronaldo de Carvalho Augusto et al.
PLoS neglected tropical diseases, 11(7), e0005789-e0005789 (2017-07-29)
Schistosomiasis has been reported in 78 endemic countries and affects 240 million people worldwide. The digenetic parasite Schistosoma mansoni needs fresh water to compete its life cycle. There, it is susceptible to soluble compounds that can affect directly and/or indirectly
Guillaume Tetreau et al.
Frontiers in immunology, 8, 1249-1249 (2017-10-21)
Detection of pathogens by all living organisms is the primary step needed to implement a coherent and efficient immune response. This implies a mediation by different soluble and/or membrane-anchored proteins related to innate immune receptors called PRRs (pattern-recognition receptors) to
Arupa Ganguly
Human mutation, 19(4), 334-342 (2002-04-05)
Conformation-sensitive gel electrophoresis (CSGE) was developed as a method of heteroduplex analysis to screen large multi-exon genes for sequence variation. The novelty of the method was in the use of a non-proprietary acrylamide gel matrix that used 1,4-bis (acrolyl) piperazine
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