D0195
Dimethylethylammoniumpropane sulfonate
≥97% (TLC)
Sinónimos:
NDSB 195
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About This Item
Fórmula empírica (notación de Hill):
C7H17NO3S
Número de CAS:
Peso molecular:
195.28
UNSPSC Code:
12161900
NACRES:
NA.25
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description
zwitterionic
Quality Level
assay
≥97% (TLC)
form
powder
mol wt
195.28 g/mol
technique(s)
LC/MS: suitable
protein quantification: suitable
color
white
application(s)
detection
Storage temp.
room temp
Categorías relacionadas
General description
Dimethylethylammoniumpropane sulfonate is a zwitterionic solubilizing agent with usefulness in protein crystallization.
Application
Dimethylethylammoniumpropane sulfonate has been used in a study to assess the functional properties of the separate subunits of the human DNA helicase II/Ku autoantigen. It has also been used in a study to investigate the swelling behaviors of copolymeric hydrogels in various saline solutions.
Non-detergent sulfobetaine is a compound used for non-denaturing protien purification. Increases the extraction yield of membrane, nuclear and cytoskeletal associated proteins. Zwitterionic over a wide pH range, easily removed by dialysis and no significant absorption in the near UV range. Specific applications include microsomal protein extraction, nuclear protein recovery, precipitation reduction in IEF, and membrane-bound protease purification.
Other Notes
This product is non-micelle forming.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Certificados de análisis (COA)
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A E Ochem et al.
The Journal of biological chemistry, 272(47), 29919-29926 (1997-12-31)
The Ku antigen consists of two subunits of 70 and 83 kDa and is endowed with both duplex DNA end-binding capacity and helicase activity (human DNA helicase II). HeLa Ku can be isolated from in vitro cultured human cells uniquely
Thermoreversible hydrogels XIII: Synthesis and swelling behaviors of [N-isopropylacrylamide-co-sodium 2-acrylamido-2-methylpropyl sulfonate-co-N,N-dimethyl(acrylamido propyl) ammonium propane sulfonate] copolymeric hydrogels
Lee, W. and W. Yuan
Journal of Polymer Research, 7, 29-29 (2000)
L Vuillard et al.
FEBS letters, 353(3), 294-296 (1994-10-24)
The potential usefulness of the new zwitterionic solubilizing agent, dimethyl ethylammonium propane sulfonate (NDSB195), in protein crystallization was shown using hen egg-white lysozyme. In the presence of this agent, highly diffracting crystals were obtained using ammonium sulphate as a precipitant
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