The kit does not have specified lower and upper detection limits for enzyme activity. The enzyme activity needs to be empirically determined using varying enzyme amounts.
CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Sinónimos:
Beta-NAG Activity Assay Kit
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| A ustedes/SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
UNSPSC Code:
12161503
EC Number:
222-398-7
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleQuality Segment
usage
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NAGLU(4669)
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
1 of 1
Este artículo | |||
|---|---|---|---|
| Gene Information human ... NAGLU(4669) | Gene Information human ... CS(1431) | Gene Information human ... HMGCR(3156) | Gene Information human ... ALPL(249) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL) | usage kit sufficient for 100 reactions (using a 1 ml cuvette), kit sufficient for 480 reactions (using 96 multiwell plates) | usage sufficient for 100 assays (200 μL), sufficient for 30 assays (1 mL) | usage kit sufficient for 300 reactions (200 μl reactions) |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
Still not finding the right product?
Explore all of our products under β-N-Acetylglucosaminidase Assay Kit
Solo componentes del kit
Referencia del producto
Descripción
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
signalword
Warning
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
target_organs
Liver,Kidney
wgk
WGK 3
Clase de almacenamiento
10 - Combustible liquids
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
What are the lower and upper limits of enzyme activity that the CS0780 B-N-Acetylglucosaminidase Assay Kit can measure?
1 answer-
Helpful?
-