C32TG

96020925, human skin, Not specified

• UNSPSC Code: 41106514

Propiedades

• product name: C32TG, 96020925
• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Amelanotic Melanoma

Cell Line Description

This cell line has been derived by the selection of the human amelanotic melanoma cell line C32 for 6-thioguanine resistance. The parent cell line C32 was established from a melanoma from a 53 year old Caucasian male. The cells are resistant to 6-thioguanine and sensitive to HAT medium. They are deficient in hypoxanthine ? guanine phosphoribosyl transferase (HGPRT-). The cell line is also known as C32-r16TG. The cells are tumorigenic in nude mice inoculated subcutaneously with 10E7 cells.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.050mM 6-thioguanine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80 %) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO₂.

Other Notes

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