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Merck

HA cl. 55

no DNA, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG2b
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Sp2/0 Ag-14
Reactivity:
Anti-Measles Virus Haemagglutinin
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biological source

Mouse x Mouse Hybridoma

packaging

tube of 5 μg 95040311-DNA-5UG, pkg of vial of cells 95040311-1VL

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0 Ag-14

Reactivity

Anti-Measles Virus Haemagglutinin

technique(s)

cell culture | mammalian: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−196°C

Immunogen

Measles virus (strain Hallé)

Application

Diagnosis, virus neutralisation.

Biochem/physiol Actions

Mouse BALB/c spleen x Mouse Sp2/0 Ag-14
The hybridoma cell line HA cl. 55 was produced by fusing myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. Immunofluorescence studies showed intracellular and membrane fluorescence with a diffuse pattern. The monoclonal antibody precipitates haemagglutinin polypeptide and neutralises measles virus infectivity. Biological assays showed haemagglutinin inhibition activity.

Preparation Note

Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].




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