LIM1215 human

10092301, human colon, Epithelial

• Sinónimos: LIM1215 Cell Line
• UNSPSC Code: 41106514

Propiedades

• product name: LIM1215 human, 10092301
• biological source: human colon
• description: Colon carcinoma from human colon
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Colorectal carcinoma from human colon

Human colorectal carcinoma

Application

For complete product information, please see ECACC

Culture Medium

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6?g/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM

Subculture Routine

Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Tony Burgess - Ludwig Institute for Cancer Research, Australia