SNL 76/7 Cell Line from mouse

7032801, mouse esophagus, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: SNL 76/7 Cell Line from mouse, 07032801
• biological source: mouse esophagus
• description: Mouse SIM strain embryonic fibroblast
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse SIM strain embryonic fibroblast

Cell Line Description

SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.

Culture Medium

Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C.

Other Notes

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