RCC4plusVHL

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 3112703, human kidney, Epithelial

• Sinónimos: RCC4 VHL(+) Cells, RCC4-pVHL Cells, RCC4/VHL Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human kidney
• envase: tube of 5 μg 03112703-DNA-5UG; pkg of vial of cells 03112703-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL

Descripción línea celular

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL. The plasmid pcDNA3-VHL confers neomycin resistance and encodes the von Hippel-Lindau (VHL) tumour suppressor gene product pVHL. The original renal carcinoma cell line RCC4 is VHL-deficient. The RCC4 plus vector alone cell line (Sigma catalogue no. 03112702) can serve as a negative control cell line to study the effect of pVHL expression from pcDNA3-VHL. These cells are less tumourigenic than corresponding cell lines lacking pVHL function.

Aplicación

Study of tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. Cell responses to hypoxia.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

Medio de cultivo

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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