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C8571

Sigma-Aldrich

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

Sinónimos:

Cathepsin B1

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About This Item

Número de CAS:
9047-22-7
Comisión internacional de enzimas:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD00130748
UNSPSC Code:
12352200
NACRES:
NA.32
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biological source

human liver

Quality Level

200

form

buffered aqueous solution

specific activity

≥1,500 units/mg protein (E1%/280)

UniProt accession no.

P07858

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSB(1508)

General description

Cathepsin B gene is mapped to human chromosome 8p23.1.[1] It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence.[2] It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.[3]

Application

Cathepsin B from human liver has been used:
  • as a positive control in the screening Cathepsin B inhibitor assay[4]
  • in wall extension (expansin activity) assay[5]
  • in microinjection human foreskin fibroblasts to test its effect on apoptosis induction[6]

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC).[1] It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.[7]
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Packaging

Package size based on protein content

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Substrate

Referencia del producto
Descripción
Precios

inhibitor

Referencia del producto
Descripción
Precios

related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000353058
0000342604
0000336414
0000328972
0000312003

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Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
Canbay A, et al.
The Journal of Clinical Investigation, 112(2), 152-159 (2003)
Amino acid sequence of human liver cathepsin B
Ritonja A, et al.
Febs Letters, 181(1), 169-172 (1985)
The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
The Embo Journal, 10(9), 2321-2330 (1991)
Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways
Wu X, et al.
Hepatology, 47(6), 1905-1915 (2008)
Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis
Gu J, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)
Ver todos los artículos relacionados

Artículos

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

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