This product is supplied as a lyophilized powder prepared from a solution containing sodium phosphate buffer salts and sodium chloride. The specific buffer formulation is considered proprietary. The pre-lyophilization fill volume is not provided. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C5275-5MG-PW0000513988.pdf
C5275
Concanavalin A from Canavalia ensiformis (Jack bean)
Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture
Sinónimos:
Con A
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Form:
lyophilized powder
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Servicio técnico
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aseptically processed
Quality Segment
type
Type IV-S
product line
BioReagent
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
impurities
salt (Contains buffer salts and NaCl)
solubility
PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy
storage temp.
−20°C
General description
Concanavalin A is a protein containing only β-sheets in its structure, and belongs to the legume lectins family.
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
- in in vitro cell proliferation assay and cell cycle assessment[1]
- in lymphocyte proliferation
- in splenocyte culturing
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Concanavalin A is known to stimulate acute hepatic inflammation and acts as a T‐cell mitogen. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy.
Analysis Note
A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form saline suspension |
| solubility PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy | solubility PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy | solubility - | solubility - |
| impurities salt (Contains buffer salts and NaCl) | impurities salt, essentially free | impurities Carbohydrate, essentially free, salt, essentially free | impurities - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
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signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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