C2888

Acetilcolinesterasa from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

• Sinónimos: AChE, Acetil colinesterasa, Acetilcolina acetilhidrolasa
• Número CAS: 9000-81-1
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-559-3
• MDL number: MFCD00081268
• Número CE: 3.1.1.7 (BRENDA, IUBMB)
• Specific activity: ≥1,000 units/mg protein
• Biological source: Electrophorus electricus

Propiedades

• biological source: Electrophorus electricus
• Quality Segment: 200
• type: Type V-S
• form: lyophilized powder
• specific activity: ≥1,000 units/mg protein
• mol wt: 280 kDa
• composition: Protein, ≥45% biuret
• pI: ~5.3
• solubility: Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
• storage temp.: −20°C

Descripción

General description

Peso molecular: 280 kDa
Punto isoeléctrico: 5,5
Coeficiente de extinción: E^1% = 18,0 (280 nm)

La acetilcolinesterasa de Electrophorus electricus es un tetrámero compuesto de 4 subunidades iguales de 70 kDa cada una. Cada subunidad contiene un sitio activo. La enzima es una glucoproteína que contiene hexosaminas.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:

• in acetylcholinesterase activity assay
• to study its effects on Xenopus embryonic hippocampal cells
• to study the effect of acetylcholine inhibitors on development and plasticity

Biochem/physiol Actions

La principal enzima degradadora de la acetilcolina in vivo. Convierte la acetilcolina + H₂o en colina + ácido acético.

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.

Physical form

Lyophilized powder containing Tris buffer salts

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Other Notes

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One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Disclaimer

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)