C2867
Citocromo c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture
Sinónimos:
Citocromo c from horse heart
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About This Item
Productos recomendados
biological source
horse heart
product line
BioUltra
assay
≥99% (SDS-PAGE)
form
powder
mol wt
12,384
storage condition
(Tightly closed Dry)
technique(s)
cell culture | mammalian: suitable
solubility
water: 10 mg/mL, dark red-brown
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
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General description
Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria.
Research area: Apoptosis
Research area: Apoptosis
Application
Se caracterizaron los sitios específicos y el grado de oxidación en el citocromo c caballo tratado con H2O2 y UV. Se sugirió que el estado de estos sitios podría utilizarse como biomarcador del entorno oxidativo de una célula.
Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.
Biochem/physiol Actions
Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C.
El citocromo c se conoce fundamentalmente como una proteína mitocondrial transportadora de electrones. La transición del citocromo c entre los estados ferroso y férrico dentro de la célula la convierte en un eficiente transportador biológico de electrones y desempeña un papel vital en las oxidaciones celulares tanto en plantas como en animales. Se considera generalmente como un catalizador universal de la respiración, formando un puente de electrones esencial entre los sustratos respirables y el oxígeno
Preparation Note
A further purification of C2506
Prepared using TCA.
Other Notes
Encontrará más información sobre el citocromo c y el transporte de electrones en www.sigma-aldrich.com/enzymeexplorer.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Los clientes también vieron
Yuning Hong et al.
Journal of the American Chemical Society, 134(45), 18713-18723 (2012-10-17)
Interactions of cytochrome c (cyt c) with cardiolipin (CL) partially unfold the protein, activating its peroxidase function, a critical event in the execution of apoptosis. However, structural features of the altered protein species in the heterogeneous ensemble are difficult to
Bradford G Hill et al.
Free radical biology & medicine, 47(6), 675-683 (2009-06-17)
Protein thiol modifications occur under both physiological and pathological conditions and have been shown to contribute to changes in protein structure, function, and redox signaling. The majority of protein thiol modifications occur on cysteine residues that have a low pK(a);
Luz P Blanco et al.
Arthritis & rheumatology (Hoboken, N.J.), 73(12), 2282-2292 (2021-05-14)
Neutrophil extracellular traps (NETs) are extracellular lattices composed of nucleic material bound to neutrophil granule proteins. NETs may play pathogenic roles in the development and severity of autoimmune diseases such as systemic lupus erythematosus (SLE), at least in part, through
J Cai et al.
Biochimica et biophysica acta, 1366(1-2), 139-149 (1998-08-26)
Mitochondrial cytochrome c (cyt c) has been found to have dual functions in controlling both cellular energetic metabolism and apoptosis. Through interaction with apoptotic protease activating factors (Apaf), cyt c can initiate the activation cascade of caspases once it is
Cytochrome c: can't live with it--can't live without it.
J C Reed
Cell, 91(5), 559-562 (1997-12-11)
Chromatograms
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