C2867

Citocromo c from equine heart

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

• Sinónimos: Citocromo c from horse heart
• Número CAS: 9007-43-6
• UNSPSC Code: 12352202
• EC Number: 232-700-9
• NACRES: NA.25
• MDL number: MFCD00130890
• Form: powder
• Assay: ≥99% (SDS-PAGE)
• Biological source: horse heart
• Mol wt: 12,384

Propiedades

• biological source: horse heart
• Quality Segment: 300
• product line: BioUltra
• assay: ≥99% (SDS-PAGE)
• form: powder
• mol wt: 12,384
• storage condition: (Tightly closed Dry)
• technique(s): cell culture | mammalian: suitable
• solubility: water: 10 mg/mL, dark red-brown
• UniProt accession no.: P00004
• storage temp.: −20°C
• Gene Information: horse ... CYCS(100053958)

Descripción

General description

Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria.

Research area: Apoptosis

Application

Se caracterizaron los sitios específicos y el grado de oxidación en el citocromo c caballo tratado con H₂O₂ y UV. Se sugirió que el estado de estos sitios podría utilizarse como biomarcador del entorno oxidativo de una célula.

Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.

Biochem/physiol Actions

El citocromo c se conoce fundamentalmente como una proteína mitocondrial transportadora de electrones. La transición del citocromo c entre los estados ferroso y férrico dentro de la célula la convierte en un eficiente transportador biológico de electrones y desempeña un papel vital en las oxidaciones celulares tanto en plantas como en animales. Se considera generalmente como un catalizador universal de la respiración, formando un puente de electrones esencial entre los sustratos respirables y el oxígeno

Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C.

Preparation Note

A further purification of C2506

Prepared using TCA.

Other Notes

Encontrará más información sobre el citocromo c y el transporte de electrones en www.sigma-aldrich.com/enzymeexplorer.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)