Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Sinónimos:

Sodium Borate Electrophoresis Buffer

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grado

Molecular Biology
for molecular biology

Nivel de calidad

200

descripción

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

Formulario

solution

concentración

10 ×

impurezas

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

temp. de almacenamiento

room temp

Descripción general

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Aplicación

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Características y beneficios

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Envase

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Precaución

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Información legal

Bionic is a trademark of Sigma-Aldrich Co. LLC

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H319,H360FD

Clasificaciones de peligro

Eye Irrit. 2 - Repr. 1B

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000371239
0000371237
0000371239
0000371237
0000279962

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Carmen Naturil-Alfonso et al.
PloS one, 7(12), e51271-e51271 (2012-12-20)
Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate
Fast short-fragment PCR for rapid and sensitive detection of shrimp viruses.
Mrotzek G, et al.
Journal of Virological Methods, 168, 262-266 (2010)
Maria Desemparats Saenz-de-Juano et al.
Biology of reproduction, 87(4), 91-91 (2012-08-03)
Studies of embryo cryopreservation efficiency have focused mainly on technical and embryo factors. To determine how a slow freezing process affects embryo and fetal development, we studied in vivo development ability after the freezing procedure by assessing blastocyst development at

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico