B0184

Bacteriorhodopsin from Halobacterium salinarum

native sequence, lyophilized powder

• Sinónimos: BR from H. salinarum, Bacterioopsin, Bacteriorhodopsin from Halobacterium halobium
• Número CAS: 53026-44-1
• UNSPSC Code: 12352202
• NACRES: NA.56
• MDL number: MFCD00130599
• Form: lyophilized powder
• Biological source: Halobacterium salinarium

Propiedades

• biological source: Halobacterium salinarium
• Quality Segment: 100
• form: lyophilized powder
• technique(s): ligand binding assay: suitable, mass spectrometry (MS): suitable
• UniProt accession no.: B0R5N9
• storage temp.: 2-8°C
• Gene Information: Halobacterium salinarium ... OE_RS05715(5953595), VNG_RS05715(144807)

Descripción

General description

Bacteriorhodopsin is the prototypical "seven-helix" transmembrane protein (with seven α-helical domains), whose study led to advances in understanding G protein-coupled receptors (GPCRs). In Halobacteria, it acts as a light-harvesting protein, producing a proton gradient across the cell wall that is then used to drive biosynthetic processes.

Bacteriorhodopsin (BR) is a covalent complex comprising bacterioopsin protein and retinal cofactor in the equimolar ratio. It corresponds to the molecular weight of 27kDa. BR belongs to the retinylidene class of proteins. It is a seven-membrane helical protein that acts as a photon-driven pump. BR can be used in studies of the folding and kenetics of β-helical proteins.

Application

Bacteriorhodopsin is of interest in the development of artificial retinas, optical associative processors, and three-dimensional memory storage devices.

Bacteriorhodopsin from Halobacterium salinarum has been used:

• in generation of droplet lipid bilayer
• as a standard in quadrupole time-of-flight (QTOF) mass spectroscopy (MS)
• in the generation of protein-detergent complex and micelles for dynamic light scattering studies

Biochem/physiol Actions

A transmembrane retinylidine protein that functions as a proton pump driven by light energy in Holobacterium.

Bacteriorhodopsin (BR) from Halobacterium salinarum acts as a proton-driven pump. BR can be used in studies of the folding and kinetics of α-helical proteins. It is thermally stable and exhibits high photoelectric and photochemical efficiency. BR exists as trimer in a hexagonal lattice. Its photocycle intermediates are exploited in bioelectronics majorly in photoelectric and photochemical applications.

Preparation Note

Aqueous suspensions may be sonicated to achieve the desired homogeneity and may be stored for a short time at a temperature of 2-8 °C or at a temperature of -20 °C without time limitation.

Wild-type bacteriorhodopsin is isolated from Halobacterium salinarum strain S9 as purple membranes.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)