Product A9594, Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mice, is mouse IgG1. Theoretically, it will be recognized by the anti-mouse IgG secondary antibody. However, Product A9594 is already conjugated with Cy3 so a secondary antibody is not needed for detection.
A9594
ANTI-FLAG® M2 monoclonal antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Sinónimos:
Anti-ddddk, Anti-dykddddk
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NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
CY3 conjugate
Clone:
M2, monoclonal
Application:
FLAG purification, IF (d)
Citations:
42
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Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
species reactivity
all
concentration
~1 mg/mL
technique(s)
FLAG purification: suitable, direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Preparation Note
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Other Notes
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Este artículo | |||
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| description clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) | description clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide) | description 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) | description clone M1, purified immunoglobulin, buffered aqueous solution |
| biological source mouse | biological source mouse | biological source mouse | biological source (Bioreactor) |
| conjugate CY3 conjugate | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal | clone M1, monoclonal |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin (Purified IgG1 subclass) | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| species reactivity all | species reactivity all | species reactivity all | species reactivity all |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Clase de almacenamiento
10 - Combustible liquids
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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A9594（FLAG M2 Cy3標識）の抗体は抗マウスIgGの2次抗体に認識されますか。
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