Alcohol Dehydrogenase from Saccharomyces cerevisiae

Name: Alcohol Dehydrogenase from <I>Saccharomyces cerevisiae</I>
Brand: SIGMA

Sinónimos:

ADH, Alcohol Dehydrogenase from yeast, Alcohol:NAD⁺ oxidoreductase

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Número CAS:

9031-72-5

UNSPSC Code:

12352204

NACRES:

NA.25

EC Number:

232-870-4

MDL number:

MFCD00081305

Número CE:

1.1.1.1 (BRENDA, IUBMB)

Biological source:

Saccharomyces cerevisiae

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Propiedades

biological source

Saccharomyces cerevisiae

Quality Segment

200

form

powder

mol wt

~150,000

packaging

vial of 25 mg

storage temp.

−20°C

Descripción

Application

Alcohol Dehydrogenase from Saccharomyces cerevisiae has been used as a gel filtration molecular weight marker/ It has also been used as a component of nine protein mixture for mass spectroscopy analysis.

Biochem/physiol Actions

ADH (alcohol dehydrogenase) is one of the first enzymes to be isolated and purified. NAD⁺is its coenzyme. Three isozymes of yeast ADH, that is, yeast alcohol dehydrogenase-1, 2 and 3 (YADH-1, -2, -3) have been identified. YADH-1 is expressed during anaerobic fermentation, YADH-2 is expressed in the cytoplasm and YADH-3 is localized to the mitochondria. A 141kDa tetramer containing 4 equal subunits. The active site of each subunit contains a zinc atom. Each active site also contains 2 reactive sulfhydryl groups and a histidine residue.

Isoelectric point: 5.4-5.8

Optimal pH: 8.6-9.0

Substrates: Yeast ADH is most active with ethanol and its activity decreases as the size of the alcohol increases or decreases. Branched chain alcohols and secondary alcohols also have very low activity.

K_M (ethanol) = 2.1 × 10^-2M
K_M (methanol = 1.3 × 10^-1M
K_M (isopropanol) = 1.4 × 10^-1M

Inhibitors: Compounds that react with free sulfhydryls, including N-alkylmaleimides and iodoacetamide.
Zinc chelator inhibitors, including 1,10-phenanthroline,
8-hydroxyquinoline, 2,2′-dipyridyl, and thiourea.
Substrate analogue inhibitors, including β-NAD analogs, purine and pyrimidine derivatives, chloroethanol, and fluoroethanol.

Extinction Coefficient: E^1% = 14.6 (water, 280 nm)

Alcohol Dehydrogenase (ADH) is an oxidoreductase and also a pyridine nucleotide-dependent dehydrogenase. It catalyzes the generation of aldehydes or ketones by reversible oxidation of alcohols. ADH in parallel also mediates the reduction of the nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺). ADH from yeast is more active than mammalian ADHs.

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Este artículo	A7011	74931	A3263
Sigma-Aldrich A8656 Alcohol Dehydrogenase from Saccharomyces cerevisiae	Sigma-Aldrich A7011 Alcohol Dehydrogenase from Saccharomyces cerevisiae	Sigma-Aldrich 74931 Alcohol Dehydrogenase	Sigma-Aldrich A3263 Alcohol Dehydrogenase from Saccharomyces cerevisiae
biological source Saccharomyces cerevisiae	biological source Saccharomyces cerevisiae	biological source -	biological source bakers yeast
form powder	form lyophilized powder (contains buffer salts)	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt ~150,000	mol wt M_w 141-151 kDa	mol wt -	mol wt ~141,000 (four subunits)
Quality Level 200	Quality Level 300	Quality Level -	Quality Level 200
packaging vial of 25 mg	packaging -	packaging -	packaging -