A7058

Anti-polihistidina monoclonal

clone HIS-1, purified from hybridoma cell culture

• Sinónimos: Monoclonal etiqueta 6xHis, Monoclonal etiqueta His, Monoclonal etiqueta His6, Monoclonal etiqueta de epítopo 6 H, Monoclonal etiqueta de epítopo HHHHHH, Monoclonal etiqueta hexa His, Monoclonal etiqueta poli-His, Monoclonal etiquetado con histidina
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: peroxidase conjugate
• Clone: HIS-1, monoclonal
• Application: WB
• Citations: 152

Propiedades

• biological source: mouse
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HIS-1, monoclonal
• form: lyophilized powder
• packaging: vial of 0.5 mL
• concentration: 5-11 mg/mL
• technique(s): western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
• isotype: IgG2a
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Descripción

General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

Proteína de fusión recombinante etiquetada con polihistidina.

Application

Antibody suitable for immunoblotting. Working dilution 1:20



Also suitable for dot blot assays and ELISA

Biochem/physiol Actions

El anticuerpo reconoce la polihistidina sintética, así como las formas reducidas, nativas o desnaturalizadas, de proteínas etiquetadas con 6 histidinas, expresadas en vectores seleccionados.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Clase de almacenamiento: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable