A6559

Adenosine 5′-triphosphate disodium salt solution

Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

• Sinónimos: ATP
• Número de CAS: 987-65-5
• Número MDL: MFCD00080339
• Código UNSPSC: 41106305
• eCl@ss: 32160414
• ID de la sustancia en PubChem: 24891106
• NACRES: NA.52

Propiedades

• Nivel de calidad: 300
• Formulario: liquid
• concentración: 100 mM in H₂O (adjusted to pH 7 with Trizma^®; base)
• actividad extraña: DNase, RNase, none detected
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• cadena SMILES: [Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
• InChI: 1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1
• Clave InChI: TTWYZDPBDWHJOR-IDIVVRGQSA-L

Descripción

Descripción general

Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.

Aplicación

Adenosine 5′-triphosphate disodium salt solution has been used:

• as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular
• as a component of the internal pipette solution to establish a recording electrode to study neurite stretching
• in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay
• as a component of lysis and resealing buffers for ATP studies
• in spectrophotometric measurement of H⁺ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells
• to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)

Acciones bioquímicas o fisiológicas

Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.

Información legal

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)