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A6017

Sigma-Aldrich

ω-Aminohexyl–Agarose

saline suspension

Sinónimos:

Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose

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About This Item

Número de CAS:
58856-73-8
MDL number:
MFCD00801286
UNSPSC Code:
23151817
NACRES:
NA.56

biological source

plant

Quality Level

200

form

saline suspension

extent of labeling

≥5 μmol per mL

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino of 1,6-diaminohexane

matrix spacer

1 atom

capacity

≥5 mg/mL binding capacity (bovine serum albumin)

suitability

suitable for chromatography

storage temp.

2-8°C

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General description

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

Application

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.

Biochem/physiol Actions

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.

Physical form

Suspension in 0.5 M NaCl containing preservative

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

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Y Ruttyn et al.
Journal of chromatography, 491(2), 299-308 (1989-07-21)
Human plasma was chromatographed under different experimental conditions on aminohexyl Sepharose. A strong retention of factor VIII/vWf and of factors VII, II, IX and X was observed. A satisfactory stabilization of eluted factor VIII clotting activity was obtained after addition
D Couchie et al.
The Biochemical journal, 199(2), 441-446 (1981-11-01)
Chromatography on hexyl-agarose resolved a partially purified cyclic GMP-activated phosphodiesterase from rat liver into two peaks of activity: the first was eluted with 0.5 M-KCl and was cyclic AMP-specific. The second was tightly bound to hexyl-agarose and was not eluted
Julio Blanco et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 2), 352-354 (2002-01-25)
Aspartokinase III catalyzes the commitment step in the aspartate metabolism pathway, the phosphorylation of aspartic acid. The Escherichia coli enzyme has been crystallized in the presence of its natural substrate (aspartic acid) and Mg-ADP and diffraction data has been collected
Hydrophobic chromatography: use for purification of glycogen synthetase.
Shaltiel S and Er-El Z
Proceedings of the National Academy of Sciences of the USA, 70, 778-781 (1973)
K Nakashima et al.
Infection and immunity, 65(9), 3794-3798 (1997-09-01)
The serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 is reported to be the O antigen of lipopolysaccharide, and the highest titers of serum antibody reactive with A. actinomycetemcomitans in early-onset periodontitis (EOP) patients bind SbAg. These high titers
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