A5588

Anti-polihistidina monoclonal

clone HIS-1, purified from hybridoma cell culture

• Sinónimos: Monoclonal etiqueta 6xHis, Monoclonal etiqueta His, Monoclonal etiqueta His6, Monoclonal etiqueta de epítopo 6 H, Monoclonal etiqueta de epítopo HHHHHH, Monoclonal etiqueta hexa His, Monoclonal etiqueta poli-His, Monoclonal etiquetado con histidina
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: HIS-1, monoclonal
• Application: WB
• Citations: 22

Propiedades

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HIS-1, monoclonal
• form: buffered aqueous glycerol solution
• technique(s): western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

Proteína de fusión recombinante etiquetada con polihistidina.

Application

Antibody is suitable for ELISA, immunoblotting and dot blotting assays.

Biochem/physiol Actions

El anticuerpo reconoce la polihistidina sintética, así como las formas reducidas, nativas o desnaturalizadas, de proteínas etiquetadas con 6 histidinas, expresadas en vectores seleccionados.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)