A3403

αα-Amilasa from Bacillus licheniformis

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

• Sinónimos: 1,4-α-D-glucano glucanohidrolasa
• Número CAS: 9000-85-5
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• Número CE: 3.2.1.1 (BRENDA, IUBMB)
• MDL number: MFCD00081319
• Specific activity: ≥500 units/mg protein (biuret)

Propiedades

• type: Type XII-A
• Quality Segment: 200
• form: saline solution
• specific activity: ≥500 units/mg protein (biuret)
• mol wt: 57.6 kDa
• sustainability: Sustainability features
• storage temp.: 2-8°C

Descripción

General description

α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)₈ structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain.
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Physical form

Aqueous solution containing sodium chloride and sucrose

Other Notes

1 U setzt 1.0 mg Maltose aus Stärke in 3 Minuten frei bei pH 6.9 und 20 °C.

Legal Information

A product of Novozyme Corp.

Disclaimer

Reported to be heat stable at temperatures as high as ∼90 °C.

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• ¿Qué está pasando?: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter