This product is not tested for RNase activity. Currently, an RNAse tested PMSF product is not available.
93482
Phenylmethanesulfonyl fluoride solution
~0.1 M in ethanol (T)
Sinónimos:
Benzylsulfonyl fluoride, PMSF
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Número CAS:
UNSPSC Code:
12352202
NACRES:
NA.77
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2088311
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Servicio técnico
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Permítanos ayudarleQuality Segment
biological source
microbial, synthetic
form
liquid
concentration
~0.1 M in ethanol (T)
density
0.797 g/mL at 20 °C
storage temp.
2-8°C
SMILES string
FS(=O)(=O)Cc1ccccc1
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
InChI key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
Application
Phenylmethanesulfonyl fluoride solution has been used as a component in the cell lysate buffer in breast adenocarcinoma cell line, fibrosarcoma cell line and D5 hindbrain and spinal cord neural progenitors.
Biochem/physiol Actions
Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.[1]
Phenylmethanesulfonyl fluoride (PMSF) is a serine protease inhibitor. It binds covalently to active site residue of proteases and prevents proteolytic activity. PMSF is used in cell lysate buffers during protein purification to prevent target protein degradation.[2] PMSF effectively inhibits the naloxone-precipitated withdrawal jumping.
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Este artículo | |||
|---|---|---|---|
| biological source microbial, synthetic | biological source synthetic | biological source - | biological source synthetic |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| form liquid | form powder or crystals | form solution | form powder |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. - |
| concentration ~0.1 M in ethanol (T) | concentration - | concentration ~50% in acetonitrile | concentration - |
| density 0.797 g/mL at 20 °C | density - | density - | density - |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2
Clase de almacenamiento
3 - Flammable liquids
wgk
WGK 2
flash_point_f
53.6 °F - closed cup
flash_point_c
12 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Is this product 93482 RNase-free?
1 answer-
Helpful?
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What can Product 93482, Phenylmethanesulfonyl fluoride solution, be used for?
1 answer-
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.
Helpful?
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How can I further dilute Product 93482, Phenylmethanesulfonyl fluoride solution?
1 answer-
We would recommend that you use an anhydrous ethanol to further dilute this solution, since PMSF is very unstable in water.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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