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49409

Sigma-Aldrich

Phalloidin-Atto 488

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sinónimos:

Atto 488, Atto 488 Phalloidin

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About This Item

UNSPSC Code:
12161900
NACRES:
NA.25

product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

λ

in water

UV absorption

λ: 499-505 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Phalloidin Atto 488is a hydrophilic fluorescent label with high water solubility. Post coupling to a substrate, the fluorescent label carries a net electrical charge of -1. The fluorescence activity is excited efficiently at the 480-515nm range. A suitable excitation source for Phalloidin Atto 488 is the 488 nm line of the Argon-Ion laser.

Application

Phalloidin Atto 488 is used for labeling F-actin in fixed cells since the binding nature is like natural phallacidin. Phalloidin conjugates identify and quantify F-actin in tissues, cell cultures, or cell-free preparations. Since fluorescent Phalloidin Atto 488 conjugates are non-permeable to live cells, it is suitable to identify cells with compromised membranes.

Features and Benefits

Characteristic features of the Phalloidin Atto 488 are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Minimal Triplet formation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Evan W Craig et al.
Molecular biology of the cell, 30(22), 2827-2837 (2019-09-19)
The green alga Chlamydomonas reinhardtii is a leading model system to study photosynthesis, cilia, and the generation of biological products. The cytoskeleton plays important roles in all of these cellular processes, but to date, the filamentous actin network within Chlamydomonas
Matthew J Fischl et al.
The Journal of physiology, 597(16), 4341-4355 (2019-07-05)
Ongoing, moderate noise exposure does not instantly damage the auditory system but may cause lasting deficits, such as elevated thresholds and accelerated ageing of the auditory system. The neuromodulatory peptide urocortin-3 (UCN3) is involved in the body's recovery from a
Daniel T Kovari et al.
Biophysical journal, 111(12), 2698-2710 (2016-12-22)
Conventional studies of dynamic phagocytic behavior have been limited in terms of spatial and temporal resolution due to the inherent three-dimensionality and small features of phagocytosis. To overcome these issues, we use a series of frustrated phagocytosis assays to quantitatively
Jaekwang Jeong et al.
PloS one, 12(4), e0174849-e0174849 (2017-04-04)
ErbB2/HER2/Neu is a receptor tyrosine kinase that is overexpressed in 25-30% of human breast cancers, usually associated with amplification of the ERBB2 gene. HER2 has no recognized ligands and heterodimers between HER2 and EGFR (ErbB1/HER1) or HER2 and ErbB3/HER3 are
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