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Merck
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49290

Sigma-Aldrich

β-Glucosidase from almonds

lyophilized, powder, ≥4 U/mg

Sinónimos:

β-D-Glucoside glucohydrolase

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About This Item

Número de CAS:
9001-22-3
Comisión internacional de enzimas:
3.2.1.21 (BRENDA, IUBMB)
EC Number:
232-589-7
MDL number:
MFCD00130628
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

quality

lyophilized

specific activity

≥4 U/mg

mol wt

Mr ~135000

storage temp.

2-8°C

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Application

β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.

Biochem/physiol Actions

βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol glucose per minute at pH 5.0 and 37 °C (salicin as substrate)

Other Notes

Role of β-glucosidase in cellulose hydrolysis: Review; Enzymic hydrolysis of cellulose for fuel ethanol production by simultaneous saccharification and fermentation; Stereospecific attachment of carbohydrates to amino acid derivatives; Enzyme-catalyzed synthesis of alkyl β-D-glucosides in organic media

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

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G P Philippidis et al.
Biotechnology and bioengineering, 41(9), 846-853 (1993-04-15)
The biochemical conversion of cellulosic biomass to ethanol, a promising alternative fuel, can be carried out efficiently and economically using the simultaneous saccharification and fermentation (SSF) process. The SSF integrates the enzymatic hydrolysis of cellulose to glucose, catalyzed by the
Turner, N.J., and Webberley, M.C.
Journal of the Chemical Society. Chemical Communications, 1349-1349 (1991)
Laura Nyström et al.
Journal of agricultural and food chemistry, 55(22), 9059-9065 (2007-10-03)
Rye and wheat bran are excellent natural sources of plant sterols in the diet. Their content, however, may vary according to processing. Thermal (roasting and heating in a microwave oven), mechanical (milling and cryogenic grinding), and enzymatic treatments (hydrolysis with
D Cantacuzene et al.
Biomedica biochimica acta, 50(10-11), S231-S236 (1991-01-01)
The transglycosylation from raffinose and lactose to Aloc-Ser-OMe is catalyzed respectively by alpha and beta galactosidases. Transglycosylation from cellobiose has been achieved with beta-glucosidase. The simplicity of the enzymatic synthesis, the stereospecificity of the condensations in one-pot reactions and the
G. Vic, and Thomas, D.
Tetrahedron Letters, 33, 4567-4567 (1992)
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