48275

β-Galactosidasa from Escherichia coli

suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg

• Sinónimos: β-D-galactósido galactohidrolasa, Lactasa
• Número CAS: 9031-11-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-864-1
• MDL number: MFCD00155434
• Número CE: 3.2.1.23 (BRENDA, IUBMB)
• Specific activity: ~140 U/mg

Propiedades

• form: powder
• Quality Segment: 100
• quality: lyophilized
• specific activity: ~140 U/mg
• mol wt: M_r ~135000
• contains: phosphate buffer as stabilizer, sucrose as stabilizer
• technique(s): enzyme immunoassay: suitable
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)

Application

Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.

Biochem/physiol Actions

βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C

Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590) ; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique ; Enzyme immunoassay of thyrptropin releasing hormone (TRH) ; Preparation of enzyme-antibody conjugates.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)