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46945

Sigma-Aldrich

Isotiocianato de fluoresceína-dextrano

average mol wt 70,000, (FITC:Glucose = 1:250)

Sinónimos:

FITC–Dextrano

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

synthetic

Quality Level

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 70,000

composition

(FITC:Glucose = 1:250)

extent of labeling

~0.004 mol/mol FITC glucose

solubility

H2O: 50 mg/mL, hazy, orange to very deep orange

fluorescence

λex 492 nm; λem 518 nm in ethanol

application(s)

cell analysis

storage temp.

2-8°C

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Application

Dextrano marcado con isotiocianato de fluoresceína para su posible uso en estudios de perfusión en animales.
Fluorescein isothiocyanate–dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-Dextran 70 may be used to study the capacity, selectivity and efficiency of encapsulation and release by drug delivery vehicles.

Other Notes

Internalización de FITC y FITC-dextrano por cultivo celular de plantas en suspensión[1]; En estudios de pinocitosis en leucocitos[2]

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Referencia del producto
Descripción
Precios

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Misty R Riddle et al.
Developmental biology, 441(2), 285-296 (2018-06-09)
Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct
L. Cole et al.
Journal of Cell Science, 96, 721-721 (1990)
Use of horseradish peroxidase and fluorescent dextrans to study fluid pinocytosis in leukocytes.
J M Oliver et al.
Methods in enzymology, 108, 336-347 (1984-01-01)
Siegfried Weisenburger et al.
Cell, 177(4), 1050-1066 (2019-04-16)
Calcium imaging using two-photon scanning microscopy has become an essential tool in neuroscience. However, in its typical implementation, the tradeoffs between fields of view, acquisition speeds, and depth restrictions in scattering brain tissue pose severe limitations. Here, using an integrated
Louise Coppin et al.
Cells, 8(8) (2019-08-10)
The efficacy of mesenchymal stem cell infusion is currently tested in numerous clinical trials. However, therapy-induced thrombotic consequences have been reported in several patients. The aim of this study was to optimize protocols for heterologous human adult liver-derived progenitor cell

Questions

  1. Is this product fixable?

    1 answer
    1. Yes, paraformaldehyde can be used to fix cells. In normal circumstances, formaldehyde is a cross-linking fixative that is used to fix protein and DNA.

      Looking at formaldehyde fixation, the following are applicable:
      Formaldehyde cross-links with the amino group of the N-terminal amino acid residue and the side chains of 12:
      Arginine
      Cysteine
      Histidine
      Lysine
      Depending on the peptide sequence, methylol groups, Schiff-bases, and methylene bridges were form

      Please note that although the term paraformaldehyde is used, paraformaldehyde exists only as a solid. Once the bonds of the polymer are broken, solutions contain only formaldehyde. Any paraformaldehyde in the solution would simply be an undissolved precipitate at the bottom of the solution.

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