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12003C

Sigma-Aldrich

Suero fetal bovino

Australia origin, USDA approved, sterile-filtered, suitable for cell culture

Sinónimos:

FBS, FCS, Suero, sueros

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About This Item

Número MDL:
MFCD00132239
Código UNSPSC:
12352207
NACRES:
NA.71
origen biológico:
bovine fetus
origen:
Australia origin
esterilidad:
sterile-filtered
Condiciones de envío:
dry ice
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

bovine fetus

Nivel de calidad

500

esterilidad

sterile-filtered

Línea del producto

FBS Premier

calidad

USDA approved

composición

Hemoglobin, ≤25 mg/dL

origen

Australia origin

técnicas

cell culture | mammalian: suitable

impurezas

≤10 EU/mL endotoxin

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Aplicación

FBS sourced in Australia is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Nota de análisis

  • Analizada la presencia de endotoxinas y hemoglobina
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Nota al pie de página

(Formerly product number F9423)

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLBR6173V
SLBR4529V

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A M Dingle et al.
Angiogenesis, 21(3), 581-597 (2018-03-28)
The liver sinusoidal capillaries play a pivotal role in liver regeneration, suggesting they may be beneficial in liver bioengineering. This study isolated mouse liver sinusoidal endothelial cells (LSECs) and determined their ability to form capillary networks in vitro and in
Qiaochu Wang et al.
Scientific reports, 9(1), 19941-19941 (2019-12-29)
Standard cytotoxic cancer treatments, such as radiation, can damage and deplete the supply of oocytes stored within the ovary, which predisposes females to infertility and premature menopause later in life. The mechanisms by which radiation induces oocyte damage have not
Shivani Sachdev et al.
PeerJ, 7, e7733-e7733 (2019-10-04)
Synthetic cannabinoids are a commonly used class of recreational drugs that can have significant adverse effects. There have been sporadic reports of co-consumption of illicit drugs with rodenticides such as warfarin and brodifacoum (BFC) over the past 20 years but
Ali Afgar et al.
Cancer biology & therapy, 17(11), 1126-1138 (2016-10-25)
It is observed that upregulation of DNMT3B enzyme in some cancers, including colon cancer, could lead to silencing of tumor suppressor genes. MiR-339 and miR-766 have been predicted to target 3'UTR of DNMT3B gene. Luciferase reporter assay validated that individual
Kate McArthur et al.
Science (New York, N.Y.), 359(6378) (2018-02-24)
Mitochondrial apoptosis is mediated by BAK and BAX, two proteins that induce mitochondrial outer membrane permeabilization, leading to cytochrome c release and activation of apoptotic caspases. In the absence of active caspases, mitochondrial DNA (mtDNA) triggers the innate immune cGAS/STING
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Artículos

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Protocolos

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Contenido relacionado

Suero bovino fetal que da la talla.

Explore nuestra gama de suero bovino fetal (FBS) y determine el FBS adecuado para sus requisitos y presupuesto de laboratorio. Solicite una muestra de suero.

Suero bovino fetal que da la talla.

Explore nuestra gama de suero bovino fetal (FBS) y determine el FBS adecuado para sus requisitos y presupuesto de laboratorio. Solicite una muestra de suero.

Fetal Bovine Serum that Makes the Grade

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Suero bovino fetal que da la talla.

Explore nuestra gama de suero bovino fetal (FBS) y determine el FBS adecuado para sus requisitos y presupuesto de laboratorio. Solicite una muestra de suero.

Ver todo

Questions

1–3 of 3 Questions  

  1. Can you provide a protocol for heat inactivation of frozen FBS?

    1 answer

    1. When handling animal serum products, it is important to follow the recommended storage, thawing, and heat inactivation procedures.Storage:Serum should not be kept in a “frost-free” freezer unit. The repeated heating cycles of the freezer unit during its self-defrosting can be harmful to serum. Recommended storage temperature is –10 to –40°C.Thawing:1. Remove serum from the freezer and allow it to sit at room temperature for 10 to 20 minutes.2. Place serum bottles in a 30 to 37°C water bath. It is very important that the temperature of the water bath not exceed 37°C. Higher temperatures may denature the proteins, potentially gelling the serum.3. Gently agitate each serum bottle every 10 minutes until completely thawed. Do not vigorously shake the bottles. Extreme agitation can cause foaming. It is very important that the serum be mixed thoroughly throughout the thawing process. Failure to agitate during the thawing process can cause the serum lipids and proteins to precipitate, resulting in turbidity or flocculence.4. It is important to remove the bottles from the water bath when completely thawed. Do not allow the bottles to remain in the water bath for an extended period. The serum should never become hot or very warm.5. When the serum is completely thawed, refrigerate promptly at 2 to 8 °C. For best results, aliquot serum into smaller, single-use sizes and return aliquots not intended for immediate use to the freezer.6. It is not uncommon for trace amounts of cryoprecipitate, fibrin, or flocculent matter to remain. This will not affect the performance of the serum. However, if one desires to eliminate these particles, it is recommended to return the serum to a 37°C water bath and gently swirl or mix it for several minutes. This will usually allow the proteins or lipids to go back into solution. This may also disappear when the serum is added to the culture media and incubated at 37 °C.Heat Inactivation:1. Prior to heat inactivation, thaw containers of serum according to the previously mentioned instructions . Allow containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Allow the bottles to return to room temperature before returning to the freezer. Failure to allow the serum to cool before freezing can cause the bottles to crack. If desirable, aliquot the serum into smaller volumes and then freeze. Sigma does not recommend repeated thaw-freeze cycles.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer

    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

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