The growth rate of these cells is subject to multiple factors, including the age and origin of the cells, growth conditions, and media. While the doubling time of these cells typically falls within a range of 22 to 46 hours, it is also not uncommon to observe a variation of up to ±10 hours beyond this range.
102-05A
Human Epidermal Keratinocytes: HEK, adult
Sinónimos:
HEK cells
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NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human neonatal foreskin or adult skin (normal)
Growth mode:
Adherent
Morphology:
Epidermal
Relevant disease(s):
allergies; cancer
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Servicio técnico
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Permítanos ayudarlebiological source
human neonatal foreskin or adult skin (normal)
Quality Segment
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 46
morphology
Epidermal
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
allergies; cancer
shipped in
dry ice
storage temp.
−196°C
General description
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Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.
Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.
Application
Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response
Biochem/physiol Actions
Skin
Preparation Note
- Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Please refer to the HEK Culture Protocol.
Other Notes
Basal Medium containing 10% FBS & 10% DMSO
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Este artículo | |||
|---|---|---|---|
| relevant disease(s) allergies; cancer | relevant disease(s) allergies; cancer | relevant disease(s) allergies; cancer | relevant disease(s) - |
| biological source human neonatal foreskin or adult skin (normal) | biological source human neonatal foreskin or adult skin (normal) | biological source human neonatal foreskin or adult skin (normal) | biological source human foreskin (juvenile epidermis) |
| growth mode Adherent | growth mode Adherent | growth mode Adherent | growth mode - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| morphology Epidermal | morphology Epidermal | morphology Epidermal | morphology ( epidermal) |
| karyotype 2n = 46 | karyotype 2n = 46 | karyotype 2n = 46 | karyotype - |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Are there any modifications applied to the 102-05A cell line from primary cells? I am conducting experiments with this cell line, but the cells are growing much faster than I expected.
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