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Merck
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03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Sinónimos:

Fluorescent Cell Counter

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About This Item

UNSPSC Code:
12352116
eCl@ss:
32161000
NACRES:
NA.32

form

liquid

usage

sufficient for 500 tests

storage temp.

−20°C

General description

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Application

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Features and Benefits

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Zhenhua Ding et al.
Medicine, 94(33), e1327-e1327 (2015-08-20)
Proteolipid protein 2 (PLP2) has been shown to be upregulated in several cancers, including breast cancer, hepatocellular carcinoma, osteosarcoma, and melanoma. PLP2 specifically binds to phosphatidylinositol 3 kinase to activate the protein kinase B pathway to enhance cell proliferation, adhesion
Zhongyi Zhou et al.
Aging, 13(7), 9838-9858 (2021-03-22)
Colorectal cancer (CRC) is a prevalent malignancy worldwide. The development of genome sequencing technology has allowed the discovery that epigenetic regulation might play a critical role in CRC tumorigenesis. In the present study, we found that the long noncoding RNA
Bin Zhou et al.
International journal of molecular medicine, 46(4), 1571-1581 (2020-09-19)
Osteoporosis is a systemic metabolic bone disease during which bone mass decreases and bone quality is reduced. Maintaining the bone formation capacity of osteoblasts is crucial for the treatment of osteoporosis. In the present study, bioinformatics analysis was performed on
Nan Zhang et al.
Oncology letters, 12(6), 4589-4597 (2017-01-21)
MicroRNA (miR)-217 has been reported to participate in carcinogenesis and tumor progression in several cancers; however, its expression and biological functions in colorectal cancer (CRC) are still unclear. The present study demonstrated that miR-217 expression was significantly higher in matched
Jianmin Li et al.
Laboratory investigation; a journal of technical methods and pathology, 101(2), 142-154 (2020-09-30)
Epithelial-to-mesenchymal transition (EMT) of epithelium and airway epithelial cell proliferation disorder are key events in idiopathic pulmonary fibrosis (IPF) pathogenesis. During EMT, epithelial cell adhesion molecules (EpCAM, such as E-cadherin) are downregulated, cytokeratin cytoskeletal transforms into vimentin-based cytoskeleton, and the
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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