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S2255

Safranin O

Name: Safranin O
Brand: SIAL

Dye content ≥85 %, Powder

Sinónimos:

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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Fórmula empírica (notación de Hill):

C₂₀H₁₉ClN₄

Número CAS:

477-73-6

Peso molecular:

350.84

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24899516

EC Number:

207-518-8

MDL number:

MFCD00011759

Colour Index Number:

50240

Beilstein/REAXYS Number:

3924099

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Propiedades

Nombre del producto

Safranin O, Dye content ≥85 %

form

powder

Quality Segment

200

composition

Dye content, ≥85%

technique(s)

microbe id | staining: suitable

pH

10 (20 °C, 10 g/L)

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

ε_max

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

Descripción

General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

Application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

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Este artículo	84120	1.04133	94635
Sigma-Aldrich S2255 Safranin O	Sigma-Aldrich 84120 Safranin O	Sigma-Aldrich 1.04133 Safranine O (C.I. 50240)	Sigma-Aldrich 94635 Gram′s safranin solution
technique(s) microbe id \| staining: suitable	technique(s) titration: suitable	technique(s) -	technique(s) microbe id \| staining: suitable
form powder	form powder or crystals	form solid	form liquid
solubility water: 1 mg/mL, clear, dark red to very dark red and red purple	solubility methanol: 0.01 g/10 mL, red	solubility 50 g/L	solubility water: soluble at 20 °C
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology microbiology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology
composition Dye content, ≥85%	composition -	composition Dye, ≥80% spectrophotometric assay	composition ethanol, 10%
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100

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pictograms

GHS05

signalword

Danger

hcodes

H318

pcodes

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–2 of 2 Questions

7 months ago

Do you have any recommendations for the storage temperature and corresponding shelf life of Safranin O after preparation?

1 answer
1. Technical Support
  
  5 months ago
  
  As Safranin O can be prepared in a variety of solvents and at varying concentrations, it is really not possible to give precise guidelines for solution stability.
  There is a Safranin O solution that is part of a Gram Stain kit (HT90A). According to the kit Instructions For Use, this solution has 0.6% dye in 20% SD3A reagent alcohol. The expiration date for this solution is 24 months. But if the formulation varies from this exact composition or if other dyes or chemicals are also present, the 24 months expiration dating may or may not be appropriate
  
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Anonymous

2 years ago

Good morning, how can i use the safranin O stain for detection of in vitro GAG ? I need the steps of preparation

1 answer
1. Technical Support
  
  2 years ago
  
  Safranin O is noted for its use in visualizing and quantifying the presence of glycosaminoglycans (GAGs) within a tissue sample. However, this application is not specifically tested, and no protocol is available to recommend. It is advised to refer to academic literature for guidance.
  
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