PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• Sinónimos: Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• Código UNSPSC: 41105323
• NACRES: NA.21

Propiedades

• Nombre del producto: Tris Buffered Saline, with 0.05% Tween^® 20, pH 8.0, powder, pHast Pack^™
• Línea del producto: pHast Pack^™
• Formulario: powder
• condiciones de almacenamiento: (Tightly closed. Dry.)
• impurezas: ≤5 ppm Heavy Metals (lead)
• color: white
• pH: 7.8—8.2
• solubilidad: soluble, clear to hazy, colorless
• trazas de catión: Fe: ≤10 ppm
• actividad extraña: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temp. de almacenamiento: room temp

Descripción

Descripción general

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

Aplicación

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

Características y beneficios

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

Envase

Foil pouches

Reconstitución

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Almacenamiento y estabilidad

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Información legal

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable