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Merck

B3301

Millipore

Bile salts

suitable for microbiology, (extracted under controlled conditions from purified fresh bile)

Sinónimos:

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85

Quality Level

biological source

(extracted under controlled conditions from purified fresh bile)

sterility

non-sterile

form

powder

loss

≤8% loss on drying

color

yellow to brownish-yellow

pH

7-9

solubility

distilled water: soluble

application(s)

microbiology

storage temp.

10-30°C

General description

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens.

Application

Bile Salts are used in MacConkey Agar and Broth as well as in Fluid Tetrathionate Medium to selectively inhibit bacteria that do not grow in an enteric environment. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.

Preparation Note

Extracted from purified, fresh bile.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Burman, N.P.
Proc. Soc. Water Treat. Exam., 10 (1955)
Martina Felici et al.
Poultry science, 99(11), 5350-5355 (2020-11-05)
The anticoccidial activity of thymol, carvacrol, and saponins was assessed in an in vitro model of coccidiosis. Eimeria spp. sporozoites were collected from field samples, characterized, and used for 2 different invasion assays on Madin-Darby Bovine Kidney cells (MDBK). The cells

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