89802
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride
derivatization grade (HPLC), LiChropur™, 98.0-102.0% (AT)
Sinónimos:
4-(4-Dimethylaminophenylazo)benzenesulfonyl chloride, DABS-Cl, Dabsyl chloride
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Fórmula lineal:
(CH3)2NC6H4N=NC6H4SO2Cl
Número CAS:
Peso molecular:
323.80
EC Number:
260-235-1
UNSPSC Code:
12352200
NACRES:
NA.22
Beilstein/REAXYS Number:
3064095
MDL number:
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derivatization grade (HPLC)
Quality Segment
assay
98.0-102.0% (AT)
form
crystals
quality
LiChropur™
technique(s)
HPLC: suitable
mp
185 °C (dec.) (lit.)
SMILES string
CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)S(Cl)(=O)=O
InChI
1S/C14H14ClN3O2S/c1-18(2)13-7-3-11(4-8-13)16-17-12-5-9-14(10-6-12)21(15,19)20/h3-10H,1-2H3/b17-16+
InChI key
VTVWTPGLLAELLI-WUKNDPDISA-N
General description
Amino groups are common to many biological compounds and many amines used in pharmaceuticals. Most of these compounds show weak absorption in direct HPLC analysis with UV-VIS detection. However, strongly absorbing derivatives of amines can be easily detected by HPLC with high sensitivity even when present at low concentrations. Primary and secondary amino groups are usually derivatized as aromatic derivatives by nucleophilic substitution reactions using reagents such as arylsulfonyl chlorides.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.
Application
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride may be used to derivatize biogenic amines, primary amino acids, melamine, and hydroxyproline and proline extracted from various biological samples prior to their determination using high performance liquid chromatography (HPLC) technique to increase their detection sensitivity.
Legal Information
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
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signalword
Danger
hcodes
Hazard Classifications
Skin Corr. 1B
Clase de almacenamiento
8A - Combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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