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63016

M-17 Agar

Name: M-17 Agar
Brand: SIAL

NutriSelect^® Basic, powder, for Lactococcus spp., for Streptococcus spp., pack of 500 g, suitable for microbiology

Sinónimos:

M17 Lactococcal Agar

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NACRES:

NA.74

UNSPSC Code:

41171605

Manufacturer/tradename:

NutriSelect^® Basic

Form:

powder

Suitability:

Lactococcus spp., Streptococcus spp.

Application(s):

agriculture
environmental
food and beverages, microbiology

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Propiedades

sterility

non-sterile

Quality Segment

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.75 g/L , ascorbic acid, 0.5 g/L , casein peptone (tryptic), 2.5 g/L , disodium β-glycerophosphate pentahydrate, 19 g/L , magnesium sulfate hydrate, 0.25 g/L , meat extract, 5 g/L , meat peptone (peptic), 2.5 g/L , soyapeptone (papainic), 5 g/L , yeast extract, 2.5 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect^® Basic

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages, microbiology

suitability

Lactococcus spp., Streptococcus spp.

Descripción

General description

M-17 Agar is a specialized culture medium designed to support the growth of lactic streptococci. It contains casein peptone, meat peptone, soya peptone, yeast extract, and meat extract, which provide carbonaceous and nitrogenous compounds, vitamin B complex, and other essential growth factors. Ascorbic acid stimulates the growth of lactic streptococci, while magnesium sulfate supplies essential ions. Disodium β-glycerophosphate pentahydrate enhances the buffering capacity of the medium, promoting the growth of lactic streptococci and selectively inhibiting lactobacilli. The medium can also be used to detect phage infection by the presence of large, distinct plaques in the host bacteria’s growth, but plaque assays require the absence of glycerophosphate, which prevents calcium precipitation necessary for bacteriophage activity.

Application

M-17 Agar media is proposed for the cultivation, detection and enumeration of Iactic streptococci in milk and dairy products. The M-17 media are superior to other comparable culture media for the cultivation of the fastidious species Streptococcus cremoris, Streptococcus diacetilactis and Streptococcus Iactis. It is also suitable for cultivation and maintenance of starter cultures for cheese and yogurt manufacturing. This medium helps in detecting streptococcal mutants which are lactose non-fermenters. M-17 Agar media may also be used for the differentiation of bacteriophages infecting lactic streptococci.

Features and Benefits

NutriSelect^®Basic is a powdered culture media offering

Qualitycontrol with growth promotion test according to industry standards
Qualityassurance acc. to ISO 9001
Cost-efficient powderedmedia

Preparation Note

Dissolve 50 g in 950 ml distilled water and sterilize by autoclaving at 121°C for 15 minutes. After cooling add 50 ml aseptic 10% lactose solution.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.15108 - M 17 agar acc. to TERZAGHI

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Este artículo	17209	69964	70157
Millipore 63016 M-17 Agar	Millipore 17209 R-2A Agar	Millipore 69964 MRS Agar	Millipore 70157 Thioglycollate Broth (USP Alternative)
description NutriSelect^® Basic, powder, for Lactococcus spp., for Streptococcus spp., pack of 500 g, suitable for microbiology	description NutriSelect^® Plus, powder, for heterotrophic bacteria, pack of 500 g, suitable for microbiology	description NutriSelect^® Basic, powder, nonselective for Lactobacillus Spp., pack of 500 g	description NutriSelect^® Plus, according to USP, IP, powder, for bacteria, for anaerobic bacteria, pack of 100 g and 500 g, suitable for microbiology
form powder	form powder	form powder	form powder
application(s) microbiology, agriculture environmental food and beverages	application(s) microbiology, bioburden testing environmental food and beverages water monitoring	application(s) agriculture bioburden testing environmental food and beverages microbiology	application(s) microbiology, food and beverages sterility testing
suitability Streptococcus spp., Lactococcus spp.	suitability nonselective for Escherichia coli, nonselective for Staphylococcus spp., nonselective for Pseudomonas spp., nonselective for Bacillus spp., heterotrophic bacteria, nonselective for Candida spp., nonselective for Salmonella spp., nonselective for Enterococcus spp., nonselective for coliforms	suitability nonselective for Lactobacillus Spp., selective by low pH for Bifidobacterium spp.	suitability nonselective for coliforms, nonselective for Bacteroides spp., nonselective for Aspergillus spp., nonselective for Bacillus spp., bacteria (microaerophilic), nonselective for Clostridium spp., anaerobic bacteria, nonselective for Staphylococcus spp., nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Candida spp.
manufacturer/tradename NutriSelect^® Basic	manufacturer/tradename NutriSelect^® Plus	manufacturer/tradename NutriSelect^® Basic	manufacturer/tradename NutriSelect^® Plus
technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) -
composition yeast extract, 2.5 g/L , casein peptone (tryptic), 2.5 g/L , disodium β-glycerophosphate pentahydrate, 19 g/L , ascorbic acid, 0.5 g/L , meat peptone (peptic), 2.5 g/L , soyapeptone (papainic), 5 g/L , agar, 12.75 g/L , meat extract, 5 g/L , magnesium sulfate hydrate, 0.25 g/L	composition dipotassium phosphate, 0.3 g/L , starch, soluble, 0.5 g/L , dextrose, 0.5 g/L , proteose peptone, 0.5 g/L , casein acid hydrolysate, 0.5 g/L , magnesium sulfate, 0.024 g/L , sodium pyruvate, 0.3 g/L , yeast extract, 0.5 g/L , agar, 15 g/L	composition yeast extract, 5 g/L , triammonium citrate, 2 g/L , sodium acetate, 5 g/L , universal peptone, 10 g/L , D(+)-glucose, 20 g/L , agar, 12 g/L , meat extract, 5 g/L , manganous sulfate monhydrate, 0.05 g/L , magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L , dipotassium hydrogen phosphate, 2 g/L	composition pancreatic digest of casein, 15.0 g/L , sodium thioglycolate, 0.5 g/L , sodium chloride, 2.5 g/L , yeast extract, 5.0 g/L , dextrose, 5.5 g/L , L-cystine, 0.5 g/L

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c