Mayer's Mucicarmine Stain Solution is used to detect epithelial mucins and mucins secreting adendocarcinomonas and to demonstrate Cryptococcus neoformans and other fungi. The staining procedure involves placing a drop of the solution on a clean slide, then preparing and observing the stained fungal culture under a microscope.
41325
Mayer′s Mucicarmine Stain Solution
suitable for microscopy
Sinónimos:
Mucicarmine Stain Solution according to Mayer
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BioChemika
Quality Segment
form
liquid
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
application(s)
food and beverages
hematology
histology
storage temp.
room temp
suitability
suitable for microscopy, fungi
General description
Most mucins contain serveral glycoproteins and can therefore be detected by stains for carbohydrates. Best carmine stain is specifically used for glycogens. Mucicarmine is an empirical stain. When applied for fungal staining, it stains the spores and hyphae as pale to dark red.
Other Notes
Composition:
Carmine 1 g, Aluminum chloride 0.5 g, water 2 ml
Carmine 1 g, Aluminum chloride 0.5 g, water 2 ml
1 of 1
Este artículo | |||
|---|---|---|---|
| suitability suitable for microscopy, fungi | suitability suitable for microscopy | suitability suitable for microscopy, Bacillus spp., Corynebacterium spp. | suitability suitable for microscopy |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| form liquid | form liquid | form liquid | form liquid |
| application(s) food and beverages | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
supp_hazards
Clase de almacenamiento
8B - Non-combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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What is the Protocol for using Mayers Mucicarmine 41325 for staining fungi?
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Please provide the protocol for histology
1 answer-
Formulation 41325 contains carmine (1 g), aluminum chloride (0.5 g), and water (2 ml). On the other hand, the formulation for HT3018 consists of certified carmine (10 g/L), aluminum hydroxide (10 g/L), and aluminum chloride (5 g/L) in ethanol (50% w/v), following Southgate's modification of Mayer's mucicarmine procedure. Dilution instructions for use are provided, indicating the need to dilute product 41325 with either 10 volumes of distilled water or preferably 50-70% alcohol just before use.
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