This kit is used for the detection of hippurate hydrolase, an enzyme produced by a number of pathogenic organisms. Hippurate is the substrate embedded into the test strips. Hippurate hydrolase produced by bacteria will hydrolyze the embedded hippurate in the test strip. One of the resulting by-products, glycine, reacts with the chromogen producing a blue/purple color, indicating a positive test. This is a qualitative test. Tests are limited to cultures taken from samples that have been incubated for at least 24 hours. See the link below to review the protocol: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/398/494/01869dat.pdf
01869
Hippurate Strips Kit
suitable for microbiology
Sinónimos:
Hippurate Strips
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Acerca de este artículo
NACRES:
NA.85
UNSPSC Code:
41171621
Technique(s):
microbe id | specific enzyme detection: suitable
Storage temp.:
2-8°C
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Servicio técnico
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Permítanos ayudarletechnique(s)
microbe id | specific enzyme detection: suitable
Quality Segment
application(s)
clinical testing
environmental
food and beverages, microbiology
storage temp.
2-8°C
suitability
Campylobacter spp., Gardnerella vaginalis, Streptococcus spp.
Application
Test for identification of hippurate hydrolase activity of group B streptococci, Campylobacter jejuni, Gardnerella vaginalis and other microorganisms.
The test is based on hydrolysis of substrate (sodium hippurate) by the bacterial enzyme hippurate hydrolase and production of benzoic acid and glycine. Glycine produced by this enzymatic reaction is detected after 24 hours incubation in reaction with chromogen (ninhydrine) and the blue-purple substance is produced.
The test is based on hydrolysis of substrate (sodium hippurate) by the bacterial enzyme hippurate hydrolase and production of benzoic acid and glycine. Glycine produced by this enzymatic reaction is detected after 24 hours incubation in reaction with chromogen (ninhydrine) and the blue-purple substance is produced.
Other Notes
The kit contains 50 Hippurate Strips (Cat. No. 92472) saturated with sodium hippurate and 350 mg Chromogen in 15 ml Diluent (Cat. No. 01875)
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Este artículo | |||
|---|---|---|---|
| description suitable for microbiology | description suitable for microbiology | description United States Pharmacopeia (USP) Reference Standard | description ≥95% (HPLC) |
| technique(s) microbe id | specific enzyme detection: suitable | technique(s) microbe id | specific enzyme detection: suitable | technique(s) - | technique(s) - |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| suitability Campylobacter spp., Streptococcus spp., Gardnerella vaginalis | suitability Gardnerella vaginalis, Campylobacter spp., Streptococcus spp. | suitability - | suitability - |
| application(s) clinical testing | application(s) clinical testing | application(s) pharmaceutical (small molecule) | application(s) - |
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
Órganos diana
Central nervous system, Respiratory system
Clase de almacenamiento
3 - Flammable liquids
Punto de inflamación (°F)
50.0 °F - closed cup
Punto de inflamación (°C)
10 °C - closed cup
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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Hello, Is this product suitable to detect if there is any hippurate in our samples? Is it a quantitative test or a qualitative test? Are there any restrictions on sample types?
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