10378445001

Chromozym PK

90% (enzymatic), powder, pkg of 20 mg

• Sinónimos: C33H39N8O6COOCH3
• Código UNSPSC: 12352204

Propiedades

• descripción: Molecular Formula: C₃₃H₃₉N₈O₆COOCH₃
• Nivel de calidad: 100
• Ensayo: 90% (enzymatic)
• Formulario: powder
• mol peso: M_r 702.9
• envase: pkg of 20 mg
• fabricante / nombre comercial: Roche
• temp. de almacenamiento: room temp

Descripción

Descripción general

Chromozym^® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

Aplicación

Use Chromozym^® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

Calidad

Contaminant: <0.5% free 4-nitraniline

Secuencia

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate

Principio

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

Nota de preparación

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información legal

Chromozym is a registered trademark of Pentapharm AG, Basel

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable