MAC139

Anti-Inntag 6 (IT6) Antibody, clone R19/4-11/15

clone R19/4-11/15, from mouse

• Sinónimos: Major hevein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: R19/4-11/15, monoclonal
• reactividad de especies: Hevea brasiliensis
• reactividad de especies (predicha por homología): all
• envase: antibody small pack of 25 μg
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso UniProt: P02877
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Pro-hevein (UniProt: P02877; also known as Major hevein) is encoded by the HEV1 gene in Hevea brasiliensis (Para rubber tree). Pro-hevein is a N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin that can inhibit fungal growth. It is known to cause allergic reaction in human. It contains one barwin domain and one chitin-binding type-1 domain. Inntag 6 IT6 is a small protein domain of Hevea brasiliensis that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Unlike the monomeric HA, Flag and MYC tags, Inntag IT6 does not increase the intrinsic variability of critical size in budding yeast cells. Structure of the Inntag IT6 is based on a packed beta-sheet conformation.

Especificidad

Clone R19/4-11/15 detecs Pro-hevein in HEK293 cells tranfected with GFP-IT6.

Inmunógeno

His-tagged recombinant peptide fragment corresponding to 43 amino acids from Hevea brasiliensis.

Aplicación

Anti-Inntag 6 (IT6), clone R19/4-11/15 is a mouse monoclonal antibody that detects the Innocuous Inntag IT6 wherein the tag protein is a fragment of Hev b 6.02 of Hevea brasiliensis. It has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: A representative lot detected Inntag 6 (IT6) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunoprecipitation Analysis: A representative lot detected Inntag 6 (IT6) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunofluorescence Analysis: A representative lot detected Inntag 6 (IT6) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Calidad

Evaluated by Western Blotting in transfected lysates from HEK293cells transfected with GFP-IT6.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 6 (IT6) in 10 µg lysate from HEK293 cells transfected with GFP-IT6.

Descripción de destino

~34.1 kDa observed; 21.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in PBS without azide.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable