MABF33

Anti-MHC class II (I-A/I-E) Antibody, clone M5/114

clone M5/114, from rat

• Sinónimos: CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-associated invariant chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), gamma chain of class II antigens, Ia antigen-
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: M5/114, monoclonal
• reactividad de especies: mouse
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_001036070
• Nº de acceso UniProt: P04441
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Cd74(16149)

Descripción

Descripción general

MHC class II-associated invariant chain (I-A/I-E), also known as Ia antigen-associated invariant chain, are single-pass type II membrane glycoproteins containing a single thyroglobulin type-I domain. They are critical components in the processing of MHC class II antigen through forming a stabilizing complex following the alpha/beta heterodimer synthesis, and orchestrating complex trafficking away from the ER compartments in which class II peptide loading occurs.

Aplicación

Detect MHC class II (I-A/I-E) using this Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 validated for use in WB, FC, IH.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC on spleen cells. (Bhattacharya, A., et al. (1981). The Journal of immunology. 127(6):2488-2496.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH on thymus tissue. (de Bont, E., et al. (1999). Int. Immunol. 11(8):1295-1306.)

Calidad

Evaluated by Western Blot in mouse spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MHC class II (I-A/I-E) in 10 µg of mouse spleen tissue lysate.

Descripción de destino

~32 kDa observed.
There are 2 isoforms produced by alternative splicing: 32 kDa Isoform Long and 24 kDa Isoform Short.

Forma física

Format: Purified

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable