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Merck
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MAB5256A5

Sigma-Aldrich

Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor™ 555 Conjugate

clone NE14, from mouse, ALEXA FLUOR™ 555

Sinónimos:

Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

100

conjugado

ALEXA FLUOR™ 555

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

NE14, monoclonal

reactividad de especies

mouse, rat, pig

reactividad de especies (predicha por homología)

human (100% sequence homology), porcine (immunogen homology)

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable

isotipo

IgG1

Nº de acceso NCBI

NP_066554

Nº de acceso UniProt

P12036

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

Descripción general

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.

Aplicación

Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.

Calidad

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.

Otras notas

Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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