CP18
Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)
liquid, clone II-4C11, Calbiochem®
Sinónimos:
Collagen Type II antibody
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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
II-4C11, monoclonal
Species reactivity:
rat, human, bovine, rabbit
Application:
—
Citations:
42
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
II-4C11, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, bovine, rabbit
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1-10 µg/mL)
Immunoblotting (1-5 µg/mL,
Paraffin Sections (1-10 µg/mL, )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COL2A1(1280)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).
Immunogen
Human
purified collagen type II
Application
Frozen Sections (1-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Paraffin Sections (1-10 µg/ml, see application references)
Immunoblotting (1-5 µg/ml, see application references)
Paraffin Sections (1-10 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I. 1993 Ann. Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307.
Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
Kivrikko, K,I. 1993 Ann. Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307.
Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
Legal Information
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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