AP187B
Goat Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed
1.1 mg/mL, Chemicon®
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UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA, WB
Citations:
9
Servicio técnico
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Permítanos ayudarlebiological source
goat
Quality Segment
conjugate
biotin conjugate
antibody form
F(ab′)2 fragment of affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
concentration
1.1 mg/mL
technique(s)
ELISA: suitable, western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Application
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications:
1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000-1:100,000 for western blotting with chemiluminescent substrates.
1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.
1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry
Optimal working dilutions must be determined by the end user.
1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000-1:100,000 for western blotting with chemiluminescent substrates.
1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.
1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry
Optimal working dilutions must be determined by the end user.
This Goat anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Rabbit IgG.
Biochem/physiol Actions
Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% Sodium Azide
RECONSTITUTION:
Reconstitute with 1.5 mL sterile distilled water.
RECONSTITUTION:
Reconstitute with 1.5 mL sterile distilled water.
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C with or without aliquotting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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