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Key Documents

ABN2300A4

Sigma-Aldrich

Neuro-Chrom Pan Neuronal Marker Antibody-Rabbit, Alexa488 conjugate

Neuro-Chrom, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 488

clone

polyclonal

species reactivity

mouse, rat

manufacturer/tradename

Neuro-Chrom

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore has developed a polyclonal antibody blend that reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody. This antibody cocktail has been validated in diverse methods, cell culture and immuno-histochemistry, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology.

Specificity

Cat. # ABN2300A4 is specific to axons (neurites), dendrites, nucleus, and the cell body of neurons.
Reactivity with other species has not been determined.

Immunogen

Epitope: Whole Neuron Marker

Application

Neuro-Chrom Pan Neuronal Marker-Rabbit, Alexa488 conjugate is an antibody targeting the Pan Neuronal Marker protein, validated for use in ICC, IHC & IF.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Routinely tested Rat E18 cortex primary neurons in Immunocytochemistry.

Physical form

Polyclonal antibody cocktail blend conjugated to Alexa Fluor 488 in PBS buffer with 15mg/mL BSA and 0.05% NaN3

Storage and Stability

Maintain at 2-8°C for up to 1 year from date of receipt.

Analysis Note

Control
Rat E18 cortex primary neuron cell culture or mouse adult brain cryosections.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Xinhua Zhan et al.
Neurology, 87(22), 2324-2332 (2016-10-28)
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Andreas C Woerner et al.
Science (New York, N.Y.), 351(6269), 173-176 (2015-12-05)
Amyloid-like protein aggregation is associated with neurodegeneration and other pathologies. The nature of the toxic aggregate species and their mechanism of action remain elusive. Here, we analyzed the compartment specificity of aggregate toxicity using artificial β-sheet proteins, as well as

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