Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

ABN1735

Sigma-Aldrich

Anti-Oxytocin Receptor

from rabbit, purified by affinity chromatography

Sinónimos:

Oxytocin receptor, OT-R

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... OXTR(5021)
mouse ... Oxtr(18430)

General description

Oxytocin receptor (UniProt P97926; also known as OT-R) is encoded by the Oxtr (also known as Otr) gene (Gene ID 18430) in murine species. Oxytocin receptor (OT-R) is widely expressed in the reproductive tract, brain, kidneys, and many other organs, where it mediates the biological functions of oxytocin (OT) in mammals. Best known for their role in lactation and parturition, OT-R and OT meidate a wide variety of physiological functions, including estrous cycle, bone formation, social behaviors, as well as regulation of energy homeostasis, including food intake and thermoregulation. Central administration of OT decreases food consumption in rats, and nesfatin (a satiety hormone) increases OT secretion. Oxt-null and Oxtr-null mice display aberrant thermoregulatory function. In addition, Oxtr-knockout mice have a morphological defect in brown adipose tissue (BAT) cells and develop obesity. Human and murine oxytocin receptors are 7-transmembrane (a.a. 39-63, 75-97, 114-135, 155-175, 203-225, 275-293, 309-331) GPCRs with their N-terminal end at the extracellular side (a.a. 1-38) and C-terminal end at the cytoplasmic side (human a.a. 332-389 and mouse a.a. 332-388).

Specificity

This polyclonal antibody specifically stained wild-type, but not Oxtr-knockout, mouse brain tissue sections (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University, U.S.A).

Immunogen

BSA-conjugated linear peptide corresponding to a sequence from mouse oxytocin receptor N-terminal extracellular domain.

Application

Anti-Oxytocin Receptor Antibody, Cat. No. ABN1735, is a highly specific rabbit polyclonal antibody, that targets Oxytocin Receptor 1 and has been tested in Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A 1:500 dilution from a representative lot detected oxytocin receptor in 10 µg of A431, HEK293, HeLa, Jurkat, MCF-7, and Raji membrane extracts.

Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected oxytocin receptor immunoreactivity in wild-type, but not Oxtr-knockout, mouse frozen brain tissue sections (Courtesy of Dr. Moses V. Chao, Langone Medical Center, New York University, U.S.A).

Quality

Evaluated by Western Blotting in human uterus.

Western Blotting Analysis: A 1:500 dilution of this antibody detected oxytocin receptor in 10 µg of human uterus tissue lysate.

Target description

~55-60 kDa observed. The target band size appears larger than the calculated molecular weight of 42.77/42.81 kDa (human/mouse) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico