ABE208

Anti-β-Catenin Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: catenin (cadherin-associated protein), beta 1, 88kDa, catenin (cadherin-associated protein), beta 1 (88kD)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, human, rat, horse
• reactividad de especies (predicha por homología): ox (based on 100% sequence homology), equine (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_001895
• Nº de acceso UniProt: P35222
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: horse ... Ctnnb1(100055241) ; human ... CTNNB1(1499) ; mouse ... Ctnnb1(539003) , Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Descripción

Descripción general

β-catenin is the founding member of the β -catenin family. This protein is comprised of 12 ARM repeats and is considered to have dual function depending on its localization. Cytoplasmic β -catenin is a critical component of E-cadherin/catening adhesion complexes and is involved in regulating cadherin-dependent cell-cell interactions.When localized to the nucleus, β -catenin serves a regulatory role by binding with transcription factors from the TCF/LEF family, activating transcription. Expression of β -catenin has been reported in a variety of hair follicle cells such as cells found in the inner and outer portions of the root sheaths, and the basal and peripheral matrix cells. It has also been observed in colon tissue. Defects in expression have been linked to the development of several tumors and cancers such as; colorectal cancer (CRC), medulloblastoma (MDB), ovarian cancer (OC), and pilomatrixoma (PTR).

Especificidad

Antibody recognizes β-Catenin at the N-terminus.

Inmunógeno

Epitope: Consensus GSK3 phosphorylation site of human β-Catenin

KLH-conjugated linear peptide corresponding to the consensus GSK3 phosphorylation site of human β-Catenin.

Aplicación

Anti-β-Catenin Antibody is a Rabbit Polyclonal Antibody for detection of β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD) & has been validated in WB, ICC & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated β-Catenin from A431 lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected β-Catenin in NIH/3T3 and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Calidad

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected β-Catenin on 10 µg of A431 cell lysate.

Descripción de destino

~86kDa observed.

Ligadura / enlace

Replaces: 04-1002

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
A431 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable