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ABC950

Sigma-Aldrich

Anti-PSAT1 Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human, mouse

reactividad de especies (predicha por homología)

rabbit (based on 100% sequence homology)

técnicas

immunocytochemistry: suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PSAT1(29968)

Descripción general

Phosphoserine aminotransferase (EC 2.6.1.52; UniProt Q9Y617; also known as Phosphohydroxythreonine aminotransferase, PSAT) is encoded by the PSAT1 (also known as NLS2, PSA, PSATD) gene (Gene ID 29968) in human. PSAT1 is a class-V pyridoxal-phosphate-dependent aminotransferase family member that catalyzes the reversible conversion of 3-phosphohydroxypyruvate and to 1-phosphoserine, as well as the conversion of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to 4-phosphonooxy-L-threonine, using, in both cases, L-glutamate as an amine donor, which is converted to 2-oxoglutarate. PSAT1 is involved in serine biosynthesis from glycolytic intermediates and PSAT1 upregulation is reported in non-small cell lung cancer (NSCLC) and colon cancer cells, while PSAT1 knockdoown is shown to suppress the proliferation of MDA-MB-468 and BT-20 breast cancer cells.

Especificidad

The immunogen sequence is 100% conserved in murine PSAT1 (UniProt Q99K85) and present in both spliced isoforms of human PSAT1 (Uniprot Q9Y617).

Inmunógeno

Epitope: Region between the two pyridoxal phosphate-binding domains.
KLH-conjugated linear peptide corresponding to a sequence within the region between the two pyridoxal phosphate-binding domains of human PSAT1.

Aplicación

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected PSAT1 in A431, HeLa, HepG2, and NIH/3T3 cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Enzymes & Biochemistry
This Anti-PSAT1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PSAT1.

Calidad

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected PSAT1 in 10 µg of Jurkat cell lysate.

Descripción de destino

~40 kDa observed. 40.42 kDa (human isoform 1; Alpha) and 35.19 kDa (human isoform 2; Beta), 40.47 kDa (mouse) calculated.

Forma física

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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M G Filippone et al.
Nature communications, 13(1), 2642-2642 (2022-05-14)
Cyclin-dependent kinase 12 (CDK12) overexpression is implicated in breast cancer, but whether it has a primary or only a cooperative tumorigenic role is unclear. Here, we show that transgenic CDK12 overexpression in the mouse mammary gland per se is sufficient

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