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Key Documents

AB9668

Sigma-Aldrich

Anti-Tau phospho Threonine 231 Antibody

Chemicon®, from rabbit

Sinónimos:

Anti-DDPAC, Anti-FTDP-17, Anti-MAPTL, Anti-MSTD, Anti-MTBT1, Anti-MTBT2, Anti-PPND, Anti-PPP1R103, Anti-TAU, Anti-tau-40

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr231)

Gene Information

human ... MAPT(4137)

General description

Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Threonine 231 is phosphorylated by GSK-3beta, cdk5 and cdk1 and has been shown to be involved in the pre-tangle process of AD.

Specificity

Tau phosphoThreonine 231. The antibody recognizes Tau pThreonine 231 in samples of recombinant human Tau treated with GSK-3beta for 45 minutes. The reactivity of the antibody is blocked with the pThreonine 231 peptide but not the non-phosphopeptide or a generic phosphoThreonine-containing peptide.

Immunogen

Synthetic peptide of amino acids surrounding the phosphoThreonine 231 site of human Tau.

Application

Anti-Tau phospho Threonine 231 Antibody detects level of Tau phospho Threonine 231 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST for one hour at room temperature. Suggested antibody dilution buffer is 1% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature..

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in Dulbecco′s PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol with 1.0 mg/mL BSA and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Alexandra Marquez et al.
Neurobiology of disease, 151, 105273-105273 (2021-01-23)
Pathological hyperphosphorylated tau is a key feature of Alzheimer's disease (AD) and Frontotemporal dementia (FTD). Using transgenic mice overexpressing human non-mutated tau (htau mice), we assessed the contribution of tau to peripheral and central neurodegeneration. Indices of peripheral small and
Ashleigh Duthie et al.
Frontiers in molecular neuroscience, 12, 163-163 (2019-07-19)
Lithium has been used for decades to treat Bipolar Disorder. Some of its therapeutic benefits may be through inhibition of Glycogen Synthase Kinase (GSK)-3. Enhanced GSK3 activity associates with development of Alzheimer's disease (AD), therefore lithium is a currently used
Olav M Andersen et al.
Cell reports. Medicine, 3(9), 100740-100740 (2022-09-14)
The established causal genes in Alzheimer's disease (AD), APP, PSEN1, and PSEN2, are functionally characterized using biomarkers, capturing an in vivo profile reflecting the disease's initial preclinical phase. Mutations in SORL1, encoding the endosome recycling receptor SORLA, are found in 2%-3%
Ahsan Habib et al.
Cell transplantation, 27(3), 438-455 (2018-03-22)
Alzheimer's disease (AD) is an age-related disorder that affects cognition. Our previous studies showed that the neuroprotective fragment of amyloid procurer protein (APP) metabolite, soluble APPα (sAPPα), interferes with β-site APP-cleaving enzyme 1 (BACE1, β-secretase) cleavage and reduces amyloid-β (Aβ)
Abhay P Sagare et al.
Nature communications, 4, 2932-2932 (2013-12-18)
Pericytes are cells in the blood-brain barrier that degenerate in Alzheimer's disease (AD), a neurological disorder associated with neurovascular dysfunction, abnormal elevation of amyloid β-peptide (Aβ), tau pathology and neuronal loss. Whether pericyte degeneration can influence AD-like neurodegeneration and contribute

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